By Louis Erhardt, David Miller (auth.), David Miller MD (eds.)
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Dormandy TL: Free radical oxidation and antioxidants, Lancet, March 25, pp 647-650, 1978. Loschen G, Azzi A, Ilohe L: Mitochondial H 20 2 formation: relationship with energy conservation. FEBS Lett 33:84-87, 1973. Leibovitz BE, Siegel BV: Aspects offree radical reactions in biological systems: Aging. J Gerontol 35 (1):45--56, 1980. Krinsky NI: Carotenoid protection against oxidation. Pure Appl Chern 51:649-660, 1979. McCord JM, Fridovich I: An enzymatic function for erythrocuprein (hemocuprein).
These effects on facility were also seen in baboon and in an assortment of non-baboon primate eyes. A year later, we published a similar series of perfusions, this time using N-ethylmaleimide as the -SH reactive agent. 5 Increases in facility of up to 20% were seen in calf eyes and even higher in some monkey eyes. In contrast to the effects with iodoacetamide, homogenates of TM from perfused calf eyes showed much lower levels of inhibition of glycolysis. Effects on facility could be clearly seen at doses that produced only 10% inhibition of lactate production from glucose.
To measure the capacity of TM to remove HzO z from aqueous humor and by selective inhibition to determine the enzymes responsible. 4. Develop measures of oxidative damage, such as changes in rubidium uptake by TM cells, using perfused eyes, isolated cell suspensions, and tissue culture. 5. To further identify and characterize the defensive enzymes in animal TM and in human, using tissue culture and where possible excised tissued. 6. The most pressing need, and the most difficult, is to develop an in vivo model of primary open-angle glaucoma.