By G. Nicolò, A. Carbone, M. Esposito, L. Santi (auth.), Professor Dr. G. Leclercq, Professor Dr. S. Toma, Professor Dr. R. Paridaens, Professor Dr. J. C. Heuson (eds.)
Several papers facing the scientific relevance of steroid hormone receptors in breast melanoma have already been released. although, no ebook has overviewed stories at the moment being performed in Europe, neither is there a sign in of the ecu facilities acting receptor assays. it's been our goal to fill those gaps. a wide majority of authors who we contacted kindly agreed to con tribute to our e-book. Papers have been grouped into six major elements in response to their contents. The abundance of medical info appropriate to receptor assays led us so as to add serious summaries to assist the reader to shape his personal reviews at the topic. we wish to thank Drs. R. J. B. King, G. Contesso, L. Santi, and E. Engelsman, who helped us during this regard. we are hoping that this publication will permit the reader to develop into accustomed to the current nation of eu steroid hormone receptor stories dedicated to breast melanoma remedy. We additionally wish that it'll advertise cooperation among ecu oncologists taken with this box of research.
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Additional info for Clinical Interest of Steroid Hormone Receptors in Breast Cancer
The gel strip could then be conveniently handled, with the cellophane sheet on one side and the paper on the other. Gel slices were easily obtained with a sharp pair of surgical scissors; the graph paper used as a template Estrogen and Progestin Receptor Analysis in Human Breast Cancer 35 could be clearly seen through the gel. The gel was routinely cut into 4-mm slices. Each gel slice was placed in a plastic vial and 5 ml Instagel added; the vials were then handled as described above. Receptor Quantitation The radioactivity in the vials was measured and the efficiency calculated according to the external standard technique.
7 (162) (86) (95) (110) (97) (105) Table 4. Effect of outliers in a five-point assay on estimates of Kd obtained by three linear plot methods. The results without outliers are expressed in arbitrary units and those with outliers are shown as per cent of result without an outlier in each case. S. R. S. R. 34 96 62 96 101 68 123 underestimate of B at a relatively low concentration. R. regression was used (Table 4). The direct linear and Woolf plots tended to give low and the Scatchard plot high estimates of K d • The three linear plots were further compared for results with 10 single points more evenly spread over a 30-fold range of ligand concentrations (Table 5).
Wallgren, B. Nordenskj61d, and S. Humla Departments of Tumor Pathology and Oncology, Karolinska Hospital, Stockholm, and Department of Oncology, Regionssjukhuset, Linkoping, Sweden Introduction The concentration of estrogen receptor protein (ER) in breast carcinoma is a useful indicator of the likelihood of response to endocrine therapy . A correlation between low receptor values and early recurrence of the carcinoma has also been shown . In most centers the ER analysis is performed on surgically removed tumor specimens.