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By Winslow S. Caughey

Biochemical and scientific facets of Hemoglobin Abnormalities

summary: Biochemical and scientific features of Hemoglobin Abnormalities

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And Schiedt, W. R. (1974) J. Am. Chem. Soc. 96, 5293-5295. Caughey, W. , Maxwell, J. C , Thomas, J. M. , O'Keeffe, D. , and Wallace, W. J. ) pp. 131-152, D. Reidel, DordrechtHolland. , Choc, M. , O'Toole, M. , and Caughey, W. S. (1977) J. Biol. Chem. 252, 5498-5508. Tucker, P. , Phillips, S. E. , Perutz, M. , Houtchens, R. , and Caughey, W. S. This volume. , and Van Gelder, B. F. (1973) Biochim. Biophys. Acta 302, 475-478. Misra, H. , and Fridovich, I. (1972) J. Biol. Chem. 247, 6960-6962. Wallace, W.

1975) FEBS Lett. 56, 39-42. , and Jones, R. T. (1975) The Johns Hopkins Med. J. 136, 132-136. Winslow S. Caughey et al. 54 DISCUSSION SCHECHTER: Why is the major absorption spectrum in the lower half of the last figure so asymmetric? CAUGHEY: The asymmetry results from subtraction of the higher frequency half of the 1951 cm'-*- band to expose the 1968 cm" 1 band; this utilizes a computer manipulation. ALBEN: The small band near 1968 cm" 1 in HbCO is produced by many different types of denaturing conditions.

S. (1974) Biochem. Biophys. Res. Comm. 61, 230-236. , and Vigneron, C. (1975) FEBS Lett. 56, 39-42. , and Jones, R. T. (1975) The Johns Hopkins Med. J. 136, 132-136. Winslow S. Caughey et al. 54 DISCUSSION SCHECHTER: Why is the major absorption spectrum in the lower half of the last figure so asymmetric? CAUGHEY: The asymmetry results from subtraction of the higher frequency half of the 1951 cm'-*- band to expose the 1968 cm" 1 band; this utilizes a computer manipulation. ALBEN: The small band near 1968 cm" 1 in HbCO is produced by many different types of denaturing conditions.

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