By Dr. med., Prof. Herbert Begemann, Dr. med., Priv.-Doz. Johann Rastetter (auth.)
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In mitosis the chromosomes are comparatively coarse. Cells 1 and 2 are "young" plasma cells (plasmoblast and proplasmocyte), 16, 17 and 21 are multinucleated, 22 and 23 mitoses. 32 5 2 1 5 4 7 14 2 Fig. 6. Basophil proerythroblasts '" 1250 x The proerythroblasts, also called pronormoblasts, are the earliest precursors of the erythroid series. They do not yet contain any haemoglobin. Their size varies between 15 and 22 ~m. The darkly basophil, often slightly hatched cytoplasm, is typical. The nucleus reveals a dense reticular chromatin structure.
The granules of eosinophil granulocytes are likewise markedly enlarged. Lymphocytes and monocytes also show granules of 1-2 Jlm diameter which are usually stained bright red. (Preparation kindly supplied by Dr. M. ) Fig. 20. g. diabetes mellitus. 52 18 19 20 Fig. 21. Eosinophil and basophil granulocytes, toxic granulation of leucocytes, Pelger anomaly of the nucleus, anomalous granulation of Alder '" 1250 x Eosinophil granulocytes (cell 1-9) develop in the same way as the neutrophils. Beginning with the stage of myelocytes they are distinguishable with certainty.
Valuation. Hb A contammg erythrocytes are recognizable as weak shadows, whereas Hb F containing erythrocytes appear bright red. This method is suitable for recognizing thalassaemia major and minor, and for demonstrating fetal erythrocytes which have entered the maternal circulation. Staining Cells in the Smear Containing Methaemoglohin (Kleibauer and Betke) detlclent red blOOd cellS are not capable of this reduction. They contain only or predominantly methaemoglobin even after several hours when in normal erythrocytes all methaemoglobin has been retransformed to oxyHb.