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By Marcus Adolphus Rothschild; Murray Oratz; Sidney S Schreiber

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G � no M on o r i bosomes ( M ) � Polyribosomes ( Pl � Subun i t s ( $ ) / I , .... 4 P = 75. 6 ------ Sucrose dens i t y grad ien t ------ 6 Proportion of runoff ribosomes and mono-ribosomes in the livers of fasted rats (Norman et al. [ 1 5]). Fig. loss of polyribosomes balanced by a sharp increase in the monomer peak, without a significant change in the abundance of native subunits. The monomeric ribosome fraction was therefore isolated from the gradient and subjected to the conditions causing dissociation of runoff ribosomes.

G � no M on o r i bosomes ( M ) � Polyribosomes ( Pl � Subun i t s ( $ ) / I , .... 4 P = 75. 6 ------ Sucrose dens i t y grad ien t ------ 6 Proportion of runoff ribosomes and mono-ribosomes in the livers of fasted rats (Norman et al. [ 1 5]). Fig. loss of polyribosomes balanced by a sharp increase in the monomer peak, without a significant change in the abundance of native subunits. The monomeric ribosome fraction was therefore isolated from the gradient and subjected to the conditions causing dissociation of runoff ribosomes.

Other Nutritional Factors Affecting Liver Protein Synthesis Two observations that tend to be misunderstood should also be mentioned. First, Sidransky et al. g. threonine or tryptophan) results in an increase in liver protein content accompanied by aggregation of hepatic polyribosomes as compared with rats force-fed a complete diet [44, 45]. These responses take many hours to develop and appear to be related to loss of protein from the muscles of animals force-fed the deficient diet, probably by providing an additional source of amino acids.

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